Oak Knoll’s AP Biology students recently took on the role of molecular biologists as they conducted a complete bacterial transformation. This advanced, college-level lab provided them with direct experience with the tools and techniques of modern genetics.

Students used plasmids containing antibiotic-resistance and glowing protein genes to observe how new traits can be introduced into bacteria. After helping the cells take in the DNA, they plated the samples on antibiotic agar to observe which bacteria survived — a straightforward, visual demonstration of how genes influence cell behavior, according to Upper School Science Teacher Anna Khan. 

When students returned to their plates, the results were unmistakable. Colonies that survived the antibiotic exposure — and in some cases glowed under fluorescent light — showed which bacteria had successfully taken in and expressed their new genes. It’s the same foundational method used in biotechnology labs to produce medicines such as insulin, Khan said.

“Students get a hands-on experience with genetic engineering instead of just learning the theory. It helps them visualize and better understand the theory behind it,” she said, adding that the students handled the challenge remarkably well.

“Most groups successfully transformed bacteria with foreign genes, which is impressive, since they are doing this technique only once and without prior experience,” she said. “This lab is normally done in biotech classes and students learn and practice many techniques prior – such as plating bacteria and working with micropipettes.”

The success of the lab also speaks to Oak Knoll’s broader commitment to helping young women see themselves as capable scientists.

“Our students demonstrate great confidence and genuine interest in science, thanks to our program. All of our classes, not only AP Biology, are hands-on and provide a relevant and engaging lab component,” she said of her nearly 40 AP Biology students this year. 

“Many of them shared that they are interested in pursuing research science, medicine, or nursing, which is why they are taking this course,” she said. “This speaks to the success of the science department in inspiring girls to engage in the sciences.”

In the end, the transformation plates didn’t just reveal which genes made it into the cells — they also reflected the curiosity, skill, and growing confidence of students learning to think and work like scientists.

This feature is part of our “Learn by Doing” series, celebrating how Oak Knoll students actively build skills, deepen understanding, and live our mission through real-world learning